Here Are Ways to make the Benedict, Fehling and Biuret solution:
Benedict's solution
Benedict's solution is used to review the content of Glucose hearts Examine A Materials (FOOD). The presence of Glucose hearts Ingredients marked WITH brick red color. How to make Benedict's solution is:
(1) dissolve 173 grams of sodium cytrate and 100 grams of sodium carbonate to 600 ml distilled hearts air.
(2) Heat Up Late, Filter later.
(3) dissolved in 17.3 grams of cupric sulfate to 150 ml of distilled air.
(4) Gradually, Add solution of cupric sulfate to solution of sodium citrate-sodium carbonate. Stir constantly.
(5) Total Volume: Add distilled water so as to achieve volume 1 L.
Biuret solution
Biuret solution Used to review hearts Examine Protein A material (FOOD).
How to create a solution Biuret Namely:
(1) A solution CuSO4 (cupric sulfate) weighing 1 gram of distilled water 99 grams. Use in separated bottles.
(2) dissolve 20 grams of NaOH air 80 grams of distilled hearts. Use in separated bottles.
How to USE: The substance will be tested first drops with NaOH solution, the new taxable income drops several Currently with CuSO4 solution. The presence of proteins characterized with purple discoloration of Material tested.
Fehling's solution
How to make:
(1) To prepare Fehling A, dissolve 34.6 grams of crystal CuSO4 (cupric sulfate) in 500 ml of distilled air.
(2) To prepare Fehling B, dissolve 77 grams of KOH in 500 ml of distilled air. Then add Potassium Sodium Tartarate as much as 175 grams, Stir Up Everything After.
(3) Fehling A and B Saved in separate bottles. Both can be blended by the same volume when used. Fehling's solution is used to review in Test reducing sugar.
Above-making General conducted laboratory solution.
The container component of above is same, complex Cu (II). Then, we can created a solution with these functions as Third Solution Above. We call it [Cu (OH) 4]. From the research we have done, in high concentrated alkaline solution, we can get the weak oxidant from this complex compound.
